Flow Fair Sampling Based on Multistage Bloom Filters

Network traffic distribution is heavy-tailed. Most of network flows are short and carry very few packets, and the number of large flows is small. Traditional random sampling tends to sample more large flows than short ones. However, many applications depend on per-flow traffic other than just large flows. A flow fair sampling based on multistage Bloom filters is proposed. The total measurement interval is divided into n child time intervals. In each child time interval, employ multistage Bloom filters to query the incoming packet’s flow whether exists in flow information table or not, if exists, sample the packet with static sampling rate which is inversely proportional to the estimation flow traffic up to the previous time interval. If it is a new flow’s first packet, create its flow information and insert it into the multistage Bloom filters. The results show that the proposed algorithm is accurate especially for short flows and easy to extend

Plant Expression of Cocaine Hydrolase-Fc Fusion Protein for Treatment of Cocaine Abuse

BACKGROUND: A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of cocaine overdose and addiction. A challenge for practical therapeutic use of this enzyme exists in the large-scale protein production and, therefore, it is interesting to identify a low-cost and feasible, sustainable source of CocH3-Fc production. RESULTS: CocH3-Fc was transiently expressed in plant Nicotiana benthamiana leaves. The plant-expressed protein, denoted as pCocH3-Fc, was as active as that expressed in mammalian cells both in vitro and in vivo. However, compared to the mammalian-cell expressed CocH3-Fc protein, pCocH3-Fc had a shorter biological half-life, probably due to the lack of protein sialylation in plant. Nevertheless, the in vivo half-life was significantly extended upon the PEGylation of pCocH3-Fc. The Fc fusion did not prolong the biological half-life of the plant-expressed enzyme pCocH3-Fc, but increased the yield of the enzyme expression in the plant under the same experimental conditions. CONCLUSIONS: It is feasible to express pCocH3-Fc in plants. Further studies on the pCocH3-Fc production in plants should focus on the development of vectors with additional genes/promoters for the complete protein sialylation and for a better yield

Mcm5 Represses Endodermal Migration through Cxcr4a-itgb1b Cascade Instead of Cell Cycle Control

Minichromosome maintenance protein 5 (MCM5) is a critical cell cycle regulator; its role in DNA replication is well known, but whether it is involved in the regulation of organogenesis in a cell cycle-independent way, is far from clear. In this study, we found that a loss of mcm5 function resulted in a mildly smaller liver, but that mcm5 overexpression led to liver bifida. Further, the data showed that mcm5 overexpression delayed endodermal migration in the ventral–dorsal axis and induced the liver bifida. Cell cycle analysis showed that a loss of mcm5 function, but not overexpression, resulted in cell cycle delay and increased cell apoptosis during gastrulation, implying that liver bifida was not the result of a cell cycle defect. In terms of its mechanism, our data proves that mcm5 represses the expression of cxcr4a, which sequentially causes a decrease in the expression of itgb1b during gastrulation. The downregulation of the cxcr4a-itgb1b cascade leads to an endodermal migration delay during gastrulation, as well as to the subsequent liver bifida during liver morphogenesis. In conclusion, our results suggest that in a cell cycle-independent way, mcm5 works as a gene expression regulator, either partially and directly, or indirectly repressing the expression of cxcr4a and the downstream gene itgb1b, to coordinate endodermal migration during gastrulation and liver location during liver organogenesis

Social media usage and SME firms' sustainability: An introspective analysis from Ghana

Social media is gradually transforming diverse business ecosystems due to the limitless capabilities they offer. Given this, emerging businesses across the globe are leveraging this innovation to improve their operations. While the literature on the usage of social media by small and medium enterprises (SMEs) is still emerging, the outcomes from existing research have not been coherent. Amid this, limited empirical evidence has been adduced on the affordance of the technology for the SME ecosystem in developing economies, particularly Ghana. Following this, this study aims to fill this research gap by investigating the impact of social media usage on the long-term sustainability of SMEs, specifically in Ghana. Using empirical data from 424 respondents who are representatives of manufacturing SMEs in Ghana, using SmartPLS techniques, the study confirmed that, generally, social media usage does not only have a positive impact on SMEs but drastically drives their motivations towards resilience and sustainability. The results further revealed a positive and significant effect of social media usage value creation, business connections and opportunities on SMEs' sustainability. This study contributes to knowledge of social media usage and sustainability from a developing country's perspective. This study offers several implications for theory and practice.Doctoral Research Innovation Fund Project of CWAS of UESTC [CXJJ2021122504]Ministry of Education of the People's Republic of China, MOE: CXJJ202112250

Acceptability and feasibility of smartphone-assisted 24 h recalls in the Chinese population

Abstract Objective To examine the acceptability and feasibility of using smartphone technology to assess beverage intake and evaluate whether the feasibility of smartphone use is greater among key sub-populations. Design An acceptability and feasibility study of recording the video dietary record, the acceptability of the ecological momentary assessment (EMA), wearing smartphones and whether the videos helped participants recall intake after a cross-over validation study. Setting Rural and urban area in Shanghai, China. Subjects Healthy adults ( n 110) aged 20–40 years old. Results Most participants reported that the phone was acceptable in most aspects, including that videos were easy to use (70 %), helped with recalls (77 %), EMA reminders helped them record intake (75 %) and apps were easy to understand (85 %). However, 49 % of the participants reported that they had trouble remembering to take videos of the beverages before consumption or 46 % felt embarrassed taking videos in front of others. Moreover, 72 % reported that the EMA reminders affected their consumption. When assessing overall acceptability of using smartphones, 72 % of the participants were favourable responders. There were no statistically significant differences in overall acceptability for overweight v. normal-weight participants or for rural v. urban residents. However, we did find that the overall acceptability was higher for males (81 %) than females (61 %, P =0·017). Conclusions Our study did not find smartphone technology helped with dietary assessments in a Chinese population. However, simpler approaches, such as using photographs instead of videos, may be more feasible for enhancing 24 h dietary recalls

Qualitative Analysis of the Evolution Behaviors of Irradiation-Induced Point Defects

Point defects created by the collisions of high-energy particles and their subsequent evolutions form the foundation for all observed irradiation effects. Qualitative analysis is performed for the local and global behaviors of the planar system of nonlinear ordinary differential equations for the point defects balance model. The results indicated that the evolution behavior for the in-pile process is qualitatively very similar to the more simple annealing process, but very different from the degenerated systems that possess analytical solutions. However, quantitatively, irradiations in the in-pile process will shift the stable node away from the defect free state and change the local behaviors. A too strong irradiation may result in a nonphysical stable node and produce amorphous states, thus making the model inadequate

A novel hyperbolic tangent sliding mode observation of vehicle lateral force fed back by longitudinal force error

Abstract The tyre lateral force control is crucial to vehicle lateral stability. Vehicle side slip and out of control can be prevented effectively by observing accurately the lateral force. Thus, a novel quasi‐sliding mode observer (QSMO) is proposed. The algorithm adopts the longitudinal tyre force error as feedback considering vehicle parameter uncertainties and without a complex tyre model. First, the on‐line verification of the algorithm was carried out by dSPACE for using the experimental data of the real vehicle linear acceleration and deceleration conditions, and comparison of experimental output with different observation algorithms. Further, the simulation under emergency obstacle avoidance conditions and the double‐line shifting conditions were conducted to verify the accuracy of the algorithm respectively. Simulation results show that the percentage errors between the tyre lateral forces from the proposed QSMO and the actual data are less than 5.35%, and the prediction accuracy of the QSMO by 38.78% is higher than that of the conventional first‐order SMO (FSMO), which indicates that the QSMO is superior to the FSMO